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De novo phasing resolves haplotype sequences in complex plant genomes
de novo phasing haplotype-resolved assembly autopolyploid chromosomal rearrangement
2023/11/28
Genome phasing is a recently developed assembly method that separates heterozygous eukaryotic genomic regions and builds haplotype-resolved assemblies. Because differences between haplotypes are ignor...
Engineering the plastid and mitochondrial genomes of flowering plants
flowering plants plastid genome mitochondrial genome CRISPR/Cas9 Engineering transcription activator-like genome editing
2023/11/16
Engineering the plastid genome based on homologous recombination is well developed in a few model species. Homologous recombination is also the rule in mitochondria, but transformation of the mitochon...
Genome-wide analysis of CpG islands in some livestock genomes and their relationship with genomic features
cattle gene networks GO GWAS KEGG mixed model SNP validation
2016/11/11
When analyzing phenotypes undergoing a complex mode of inheritance, it is of great interest to switch the scope from single genes to gene pathways, which form better defined functional units. We used ...
摘要用碱处理水稻嫩叶，取碱处理水稻叶片浸出液直接用作PCR扩增的模板，扩增结果稳定、准确，与常规提取的DNA扩增效果相比没有显著差异。用此方法制备的模板室温下2周之内、4℃下3周之内、-20℃下4个月以上扩增结果不变。此方法所需试剂均为常规试剂，具有需要材料少、成本低、简便、快捷等优点，尤其适合材料比较宝贵的转基因水稻的预筛选和大样本量的PCR检测，为分子标记技术的实际应用创造了条件。
Abst...