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De novo phasing resolves haplotype sequences in complex plant genomes
de novo phasing haplotype-resolved assembly autopolyploid chromosomal rearrangement
2023/11/28
Genome phasing is a recently developed assembly method that separates heterozygous eukaryotic genomic regions and builds haplotype-resolved assemblies. Because differences between haplotypes are ignor...
Engineering the plastid and mitochondrial genomes of flowering plants
flowering plants plastid genome mitochondrial genome CRISPR/Cas9 Engineering transcription activator-like genome editing
2023/11/16
Engineering the plastid genome based on homologous recombination is well developed in a few model species. Homologous recombination is also the rule in mitochondria, but transformation of the mitochon...
Bat Biology, Genomes, and the Bat1K Project: To Generate Chromosome-Level Genomes for all Living Bat Species
echolocation, flight, longevity, immunity, ecosystem, mammals
2018/3/5
Bats are unique among mammals, possessing some of the rarest mammalian adaptations, including true self-powered flight, laryngeal echolocation, exceptional longevity, unique immunity, contracted genom...
Genome-wide analysis of CpG islands in some livestock genomes and their relationship with genomic features
cattle gene networks GO GWAS KEGG mixed model SNP validation
2016/11/11
When analyzing phenotypes undergoing a complex mode of inheritance, it is of great interest to switch the scope from single genes to gene pathways, which form better defined functional units. We used ...
Genomes of Sea Microbes
Sea Microbes Genomes
2015/7/22
Genomics is the study of the genetic information encoded by all the nucleotides possessed by an organism. For oceanographers, genomics provides detailed information on the many genes that drive biogeo...
OperomeDB: database of condition specific transcription in prokaryotic genomes and genomic insights of convergent transcription in bacterial genomes
Genomics Gene order Transcriptional interference
2015/5/26
My thesis comprises of two individual projects: 1) we have developed a database for operon prediction using high-throughput sequencing datasets for bacterial genomes. 2) Genomics and mechanistic insig...
Diversity of Eukaryotes and Their Genomes
Foraminifera Genome Evolution Microbial Eukaryotes Molecular Systematics Taxon sampling Tree of Life
2014/12/18
My dissertation addresses two aspects of eukaryotic evolution, 1) the organization of eukaryotic diversity and 2) genomic variation in Foraminifera. The bulk of eukaryotic diversity is microbial with ...
Estimation of Interaction Effects among Nucleotide Sequence Variants in Animal Genomes
Animal Genomics Bayesian Inference Epistasis Gibbs Sampling Single Nucleotide Polymorphism
2016/4/28
Estimating genetic interaction effects in animal genomics would be one of the most challenging studies because the phenotypic variation for economically important traits might be largely explained by ...
摘要用碱处理水稻嫩叶,取碱处理水稻叶片浸出液直接用作PCR扩增的模板,扩增结果稳定、准确,与常规提取的DNA扩增效果相比没有显著差异。用此方法制备的模板室温下2周之内、4℃下3周之内、-20℃下4个月以上扩增结果不变。此方法所需试剂均为常规试剂,具有需要材料少、成本低、简便、快捷等优点,尤其适合材料比较宝贵的转基因水稻的预筛选和大样本量的PCR检测,为分子标记技术的实际应用创造了条件。
Abst...
Distribution of Triplet Separators in Bacterial Genomes
bacterial genomes DNA sequence analysis
2007/8/15
2001Vol.36No.1pp.115-118DOI:
Distribution of Triplet Separators in Bacterial Genomes
HU Rui and ZHENG Wei-Mou
Institute of Theoretical Physics, Academia Sinica, Beijing ...
Genome Size Evolution in Pufferfish: A Comparative Analysis of Diodontid and Tetraodontid Pufferfish Genomes
Genome Size Evolution Diodontid and Tetraodontid
2015/12/16
Smooth pufferfish of the family Tetraodontidae have the smallest vertebrate genomes yet measured. They have
a haploid genome size of ~400 million bp (Mb), which is almost eight times smaller than the...
Androgenesis: The Best Tool for Manipulation of Fish Genomes
Androgenesis Common carp Genome conservation
2010/3/30
Androgenesis involves the development of an organism with only paternal chromosomes. The induction of androgenesis using diploid sperm, highly effective UV-irradiation of egg, dispermy using heterolog...