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Physiologic attributes of the luteinizing hormone pulse signal in the human. Cross-validation studies in men
Physiologic attributes luteinizing hormone pulse signal human
2009/2/23
The performance of two new statistically based, independently formulated, endocrine peak detection methodologies (Cluster and Detect) were tested on the same large set of physiologic LH pulsations. ...
Validation of a Direct Radioimmunoassay for Testosterone in Unextracted Serum from Five Species: Application to Study of the Hypothalamic-Pituitary-Gonadal Axis in Males
direct radioimmunoassay serum testosterone luteinizing hormone releasing hormone immunoneutralization
2009/2/19
A method for the radioimmunoassay of serum testosterone that does not require extraction or chromatography is described. Utilization of a highly specific antiserum, tritiumlabeled ligand, and doub...
Validation of an acrosomal stain for equine sperm that differentiates between living and dead sperm
acrosomal stain equine sperm living and dead sperm
2009/2/12
An acrosomal staining technique that can differentiate between living and dead sperm was developed for equine sperm. The fluoresceinated lectin Pisum sativum agglutinin (FITC-PSA) was used to iden...
Validation of an improved in vitro bioassay to measure LH in diverse species
LH bioactivity cell line bioassay diverse species
2009/2/12
Cultured Leydig tumor cells (MA-10) respond to luteinizing hormone (LH) by synthesizing and secreting progesterone (P). The specificity of the response to LH prompted us to develop this system for u...
Marked species specificity of guinea pig luteinizing hormone：validation of a bioassay
species specificity guinea pig validation of a bioassay
2008/12/26
The Guinea pig is a potentially interesting alternative small animal model for the study of the neuroendocrine regulation of reproduction. However, appropriate gonadotropin assays are not widely ava...
Validation of Sperm Counting Methods Using Limits of Agreement
Sperm Counting Methods Limits of Agreement
2008/12/24
A variety of methods exist for counting sperm. Since the introduction of semen analysis, one of these methods, the hemocytometer, has been regarded as the gold standard by andrology laboratories and t...
Measurement of Volume Changes in Mouse Spermatozoa Using an Electronic Sizing Analyzer and a Flow Cytometer: Validation and Application to an Infertile Mouse Model
Coulter counter c-ros knockout mice hypo-osmotic swelling regulatory volume decrease
2008/12/5
The importance of sperm volume has recently been highlighted in a knockout mouse model in which infertility was caused by defects in volume reguiation, which led to sperm transport failure in the fe...