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Phosphatidylcholine synthesis in human spermatozoa
Phosphatidylcholine synthesis human spermatozoa
2009/2/23
To clarify the mechanism of phospholipid synthesis in spermatozoa, fresh human spermatozoa were incubated with labeled fatty acids and 1-acyl-lysophosphatidyl choline (LPC) in the presence or abse...
Cytochalasin D inhibits penetration of hamster eggs by guinea pig and human spermatozoa
Cytochalasin D inhibits penetration hamster eggs guinea pig human spermatozoa
2009/2/23
Fertilization experiments using zona-free hamster eggs and spermatozoa from both guinea pig and human were conducted in the presence of cytochalasin D to evaluate the possible role of actin filame...
Apparent fertility of human spermatozoa from the caput epididymidis
Apparent fertility human spermatozoa caput epididymidis
2009/2/23
Fifty-one patients with obstructive azoospermia caused by blockage at the caput epididymidis have been followed for 4 years after undergoing "specific tubule" vasoepididymostomy, bypassing the cor...
A simple, clinical assay to evaluate the acrosin activity of human spermatozoa
acrosin activity human spermatozoa
2009/2/23
Acrosin, a sperm-specific acrosomal proteinase, has an essential role in the fertilization process. Low levels of acrosin appear to be associated with subfertility and infertility, and the acrosin a...
Ethanol accelerates acrosomal loss in human spermatozoa
Ethanol accelerates acrosomal loss human spermatozoa
2009/2/23
The effects of ethanol on the loss of the human sperm acrosome, as determined by the chlortetracycline fluorescence assay and by indirect immunofluorescence assay, were assessed over 6 hours during ...
Correlation between defective motility（asthenospermia）and ATP reactivation of demembranated human spermatozoa
Human spermatozoa motility asthenospermia
2009/2/23
Human spermatozoa treated with 0.05% Triton X-100 to remove the cell membranes became immotile but flagellar movement was reinitiated by addition of 0.06 to 1 mM adenosine triphosphate (ATP). The pe...
Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity
Human spermatozoa liquid peroxidation superoxide production hydrogen peroxide production
2009/2/23
Spontaneous lipid peroxidation in washed human spermatozoa was induced by aerobic incubation at 32 C and measured by malonaldehyde production; loss of motility during the incubation was determined s...
Cryosurvival of human spermatozoa frozen in eight different buffer systems
human spermatozoa eight different buffer systems
2009/2/23
The present study was conducted to ascertain optimal cryoconditions for human spermatozoa by comparing the relative cryoprotective efficiency of eight buffer systems and assessing various cryovial...
Nitrocellulose and polyvinyl coatings prevent sperm adhesion to glass without affecting the motility of intact and demembranated human spermatozoa
Nitrocellulose polyvinyl coatings sperm adhesion glass without
2009/2/23
The effectiveness of several glass coating agents in preventing sperm adherence to glass surfaces for both intact and demembranated human spermatozoa was investigated. These agents included bovine s...
Kinetics of human spermatozoa long-chain fatty acid：CoASH ligase
human spermatozoa long-chain fatty acid CoASH ligase
2009/2/23
The kinetics of long-chain saturated fatty acid activation were studied in the supernatant obtained from Triton-treated human spermatozoa. Sperm long-chain fatty acid: CoASH ligase (AMP) (E.C. 6.2.1...
Activation of palmitic acid by human spermatozoa
Activation palmitic acid human spermatozoa
2009/2/23
Human spermatozoa were studied to determine if a long chain fatty acid, CoASH ligase (AMP) (E.C. 6.2.1.3), was present. Ligase activity was measured with a radioligand millipore filter technique and...
Comparison of the penetration ability of human spermatozoa into bovine cervical mucus and zona-free hamster eggs
penetration ability human spermatozoa bovine cervical mucus zona-free hamster eggs
2009/2/20
In vitro bovine cervical mucus (BCM) penetration tests, sperm penetration assays (SPA) using zona-free hamster eggs, and routine semen analyses were performed on a total of 136 freshly collected sem...
Release, extraction, and stability of hyaluronidase associated with human spermatozoa
spermatozoa hyaluronidase human cryopreservation
2009/2/20
In contrast to the rabbit: 1) no hyaluronidase could be detected in fresh or frozen human seminal plasma, all color formation on assay being due to a dialyzable, heat stable factor; 2) almost no hya...
Effects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa
white blood cells zona-free hamster eggs human spermatozoa
2009/2/20
The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of no...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...