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利用反转录聚合酶链式反应(RTPCR)从猪囊尾蚴中克隆到18 ku蛋白基因,将扩增产物与pGEMT Easy载体连接后测序分析。将目的基因亚克隆至表达载体,构建重组质粒pGEXCE18,经转化大肠杆菌BL21(DE3)后诱导表达,用SDSPAGE和Westernblot分析表达产物。表达的目的蛋白纯化后作抗原建立检测猪囊虫抗体的重组蛋白间接ELISA方法。结果表明,18 ku 蛋白基因在...
Study of Inter-Annual Ku-Band Backscatter Variations of Amery Ice Shelf, East Antarctica
Amery Ice-shelf Ku-band Scatterometer Climate Change
2016/4/11
The widespread retreat of glaciers can be considered as response to the climate change. Being the largest retreating glacier-ice shelf system in East Antarctica, the Amery Ice Shelf-Lambert Glacier sy...