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莴苣中两种致泻性大肠杆菌的富集及DNA提取方法的优化
鲜活农产品 致泻大肠杆菌 富集 DNA提取
2016/5/27
本研究对人工接种肠出血性大肠杆菌O157∶H7和肠侵袭性大肠杆菌的莴苣样品中菌体的富集和DNA提取方法进行了优化。研究中比较了不同过滤膜组合对菌体的富集效果,筛选了莴苣样品中细菌DNA提取的最适方法,建立了多重PCR方法检测人工污染莴苣样品。结果表明,采用尼龙膜15μm+混合膜0.22μm的组合富集细菌、试剂盒法提取样品中细菌DNA,多重PCR检测的检出限降低10倍,检测时间节省1.5 h。本研究...
Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation
Lactococcus lactis subsp .lactis screening nisin gene PCR probe DNA/DNA hybridisation
2014/3/10
n this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as ...
Present and future applications of DNA technologies to improve beef production
Molecular genetics Quantitative trait loci SNP DNA Whole-genome selection
2012/8/13
Substantial improvements in production efficiency and quality of beef and dairy products have been made possible through manipulation of bovine genetics. The advent of modern breeds in the last two ce...
Design and Synthesis of α-Glucosidase Inhibitor Having DNA Cleaving Activity
α-glucosidase inhibitor DNA cleavage apoptosis ER stress
2008/4/20
Apoptosis, or programmed cell death, is a mechanism by which cells undergo death to control cell proliferation or in response to DNA damage. The present study was designed to explore small molecule ap...