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Fermentation Festival Leads to Rapid Response System at Center for Microbiome Innovation
Fermentation Festiva Response System at Center Microbiome Innovation
2016/5/6
The personalized collections of microorganisms and molecules found in, on and around us — known as our microbiome and metabolome, respectively — are increasingly recognized for their influences on eve...
CRISPR-Cas adaptive immune systems in prokaryotes boast a diversity of protein families and mechanisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recogniti...
A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
Delineation Paralleled Genotyping
2015/4/1
Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important
food and feed grain crop legume, they have not yet been used for comprehensive molecula...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
Rapid and Accurate Protein Side Chain Prediction with Local Backbone Information
side chain prediction local backbone features multiclass
2012/11/30
High-accuracy protein structure modeling demands accurate and very fast side chain prediction since such a procedure must be repeatedly called at each step of structure refinement. Many known side cha...
苦瓜MAP30基因原核表达载体的构建和PCR快速检测重组子的研究 Construction of Prokaryotic Expression Vector for MAP30 Gene and Study of PCR Methods for Rapid Identification of Recombinant
MAP30 PCR 克隆子筛选 苦瓜
2008/1/8
摘要
根据已报道的序列，把苦瓜(Momordica charantia) MAP30基因成功克隆到原核表达载体pET28a（+）中，并对PCR快速检测阳性克隆进行了研究。结果表明，直接用菌落、菌液、酚氯仿处理过的菌液,以及提取的质粒进行PCR都可以成功地筛选阳性菌落。其中，酚氯仿处理过的菌液PCR与质粒PCR的结果最接近，而且比质粒PCR简单，因此可作为方便可靠的阳性克隆筛选的新方法。Abstr...