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Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
SRY Gene Bovine Spermatozoa In situ Hybridization Bovine
2016/5/9
The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR expe...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...