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Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells
c-FLIP(L) pAcGFP-N l Recombinant Plasmid Follicular Granulosa Cell
2016/5/4
FLICE inhibitory protein (FLIP) is one of the important anti-apoptotic proteins in the Fas/FasL apoptotic path which has death effect domains, mimicking the pro-domain of procaspase-8. To reveal the i...
Improving Soluble Expression of β-Galactosidase in Escherichia coli by Fusion with Thioredoxin
Galactosidase Thioredoxin Inclusion Body IPTG
2016/4/18
Recombinant heterologous proteins can be produced as insoluble aggregates partially or perfectly inactive in Escherichia coli. One of the strateges to improve the solubility of recombinant proteins is...
Expression of Human Serum Albumin in Milk of Transgenic Mice Using Goat β-casein/Human Serum Albumin Fusion Gene
Human Serum Albumin Transgenic Mouse Gene Expression
2016/4/15
The gene encoding human serum albumin (HSA) was cloned from human liver cDNA library by PCR. The HSA cDNA in size of 2,176 bp, including 1,830 bp of open reading frame, was cloned into the plasmid car...