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Green Chem. 和J. Agric. Food Chem. 报道华东理工大学在体外生物制造领域的新进展（图）
华东理工大学 Green Chem. J. Agric. Food Chem. 体外生物制造
2020/8/31
近日，华东理工大学生物反应器工程国家重点实验室李志敏教授课题组应用多酶组合催化体系，在无细胞生物合成方向取得最新进展。有关成果分别在Green Chemistry以“Towards the conversion of CO2 into optically pure n-carbamoyl-l-aspartate and orotate by an in vitro multienzyme casc...
Green Algae Could Hold Clues For Engineering Faster-Growing Crops（图）
Green Algae Hold Clues Engineering Faster-Growing Crops
2017/10/24
Two new studies of green algae — the scourge of swimming pool owners and freshwater ponds — have revealed new insights into how these organisms siphon carbon dioxide from the air for use in photosynth...
Heme,a Poisonous Nutrient,Tracked by‘Green Lantern’Sensor
Heme Poisonous Nutrient Tracked Green Lantern Sensor
2016/6/12
In minuscule amounts, it works in cells as an essential catalyst called a cofactor and as a signaling molecule to trigger other processes. Now, for the first known time, researchers have tracked those...
Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP)
Short Hydrogen Bonds Proton Delocalization Green Fluorescent Protein GFP
2016/5/25
Short hydrogen bonds and specifically low-barrier hydrogen bonds (LBHBs) have been the focus of much attention and controversy for their possible role in enzymatic catalysis. The green fluorescent pro...
Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)
Mitotic centromere-associated kinase (MCAK/Kif2C) Cellular senescence p53 Human primary cells
2016/5/25
Proton transfer plays an important role in the optical properties of green fluorescent protein (GFP). While much is known about excited-state proton transfer reactions (ESPT) in GFP occurring on ultra...
Light-Activated Reassembly of Split Green Fluorescent Protein
Tungsten particle Cobalt-cemented tungsten carbide Adhesion Gap Diamond film
2016/5/25
Truncated green fluorescent protein (GFP) with the 11th β-strand removed is potentially interesting for bioconjugation, imaging, and the preparation of semisynthetic proteins with novel spectroscopic ...
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncate...
Deconstructing Green Fluorescent Protein
Green Fluorescent Proteins Spectrometry, Fluorescence Protein Structure,Secondary Protein Folding Peptide Fragments Models,Molecular
2016/5/25
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding t...
Dynamic Stokes Shift in Green Fluorescent Protein Variants
GFP solvation response ultrafast
2016/5/25
Solvent reorganization around the excited state of a chromophore leads to an emission shift to longer wavelengths during the excited-state lifetime. This solvation response is absent in wild-type gree...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.2.Unusual Photophysical Properties
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e
2016/5/25
In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, an...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Polarized Absorption Spectra of Green Fluorescent Protein Single Crystals:Transition Dipole Moment Directions
Animals Scyphozoa Protons Threonine Serine Luminescent Proteins Green Fluorescent Proteins Microscopy, Fluorescence Amino Acid Substitution Spectrometry, Fluorescence
2016/5/24
Polarized absorption spectra of orthorhombic crystals of wild-type green fluorescent protein (GFP) were measured between 350 and 520 nm to obtain information on the directions of the electronic transi...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...