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生命活动往往是多种细胞协同参与、时空高度调控的复杂过程。因此,对活体中的特定细胞及特定生命分子进行原位的标记、操纵及分析,是研究现代生物学问题的关键途径。基因工程方法(如融合荧光蛋白标签)能够在活细胞中提供精准的标记信号,但其应用范围受限,不适用于一些难以转染的样品(如原代细胞、临床样品等)。而近年来发展起来的生物正交反应能通过化学手段在生命活体中进行原位研究,为生物学研究提供了简单高效的工具。特...
The rubberwood samples were treated with 0.5–10% maleic anhydride (MA) solutions. The treatment of wood with 2.5% MA was adequate to prevent the growth of moulds on wood for 1 year at least. The viabl...
Currently there is considerable interest in the potential health benefits of oil seeds, such as soy and flaxseed, especially in relation to cardiovascular disease and cancer.
A micropropagation protocol was developed by further improvement of prevailing methods using proven germplasm for ex vitro rooting and addressed the effect of the number of subcultures on the rooting ...
This paper provides a prospective and retrospective quantitative assessment of the impact of a passive vertical integration between a large electricity retailer and a large electricity generator i...
Ethnopharmacological relevance: Ajuga bracteosa wall ex. Benth (Labiatae) is traditionally used medicine in the treatment of malaria and gout. The plant is substitute of cinchona. Its allied species A...
Ex vivo digestion of proteins and fat in Red Chittagong Cattle milk from Bangladesh was carried out using human gastrointestinal enzymes. This was done to investigate the protein digestion in this bov...
This paper upgrades generic and partial information from parasitological research for farm-specific decision support, using two methods from managerial sciences: partial budgeting and frontier analysi...
The aim of the study is to evaluate the role of the Grand fir (Abies grandis [Dougl. ex D. Don] Lindl.) as a soil-improving species in the area of the University Training Forest in Kostelec nad 萫rn齧i ...
Th e paper aims to assess the impact of the Common Agricultural Policy reform (post 2014) in the environmental vulnerable areas from Romania. It proposes a bio-economic dynamic farm model calibrated...
The paper presents first positive results of the experiment with three-year outdoor cultivation of Cypripedium calceolus L. ex vitro seedlings in the Czech Republic. They were propagated in vitro from...
The influence of growth regulators on in vitro bulblet formation from ex vitro roots was studied in asiatic and oriental hybrids of Lilium The root segments (3–4 mm) isolated from the middle zone of 2...
The objective of this paper was a screening of flavonoid content in Achillea collina Becker ex. Rchb. Alba flowering tops (Herba millefolii) and flowers (Flos millefolii). Ten developmental stages of ...

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