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Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
human β-galactosidase lysosomal storage diseases Pichia pastoris protein expression protein purification biochemistry and molecular biology
2014/12/12
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported...
Structural And Biochemical Studies Of The Human Lysosomal Enzymes: N-Acetylgalactosamine-6-Sulfatase, N-Sulfoglucosamine Sulfohydrolase And Beta-Galactosidase
Pure sciences Biological sciences Cysteine modification Enzyme replacement therapy Lysosomal storage disease Pharmacological chaperone therapy Sulfatase structure X-ray crystallography
2014/12/10
Lysosomal storage diseases are disorders caused by deficiencies of enzymes responsible for the degradation of substances present in lysosomes. The loss of activity of a lysosomal enzyme leads to the a...
Immobilization of the Antarctic Bacillus sp. LX-1 α-Galactosidase on Eudragit L-100 for the Production of a Functional Feed Additive
α-Galactosidase Bacillus Eudragit L-100 Immobilization α-Galacto-oligosaccharides Feed Industry
2016/5/11
Partially purified α-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtaine...
Effects of α-Galactosidase Supplementation on Performance and Energy Metabolism for Broilers Fed Corn-non-dehulled Soybean Meal Diets
a-Galactosidase Metabolizable Energy Broilers Performance Non-dehulled Soybean Meal
2016/5/5
To study the effects of α-galactosidase (α-Gal) supplementation on performance and energy metabolism, 216 Arbor Acres male broilers were placed in 36 cages of 6 birds each and allotted to 4 diets for ...
Galactose Depletion by Mortierella vinacea α-Galactosidase II Increases the Synergistic Interaction between Guar Gum and Xanthan Gum
Mortierella vinacea α-galactosidase guar gum
2009/7/7
α-Galactosidase II from Mortierella vinacea releases galactose from guar gum. This enzyme was easily prepared from the culture filtrate free of β-mannanase activity. Galactose-depleted guar gum sampl...
Characterization of Catalytic Phenotype of b-Galactosidase From LacI Mutant,E.Coli CSH-36,as a Tool For The Management of Lactose Intolerance
lactose intolerance b-Galactosidase E.coli purification kinetics
2009/6/30
Objectives: Lactose intolerance is a common public health problem with 5-100% prevalance among different populations of the world. Use of b-galactosidase to reduce the lactose content in food or consu...
Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
α-galactosidase Streptomyces coelicolor A3(2) glycoside hydrolase
2009/6/10
An α-galactosidase gene belonging to glycoside hydrolase family 27 from Streptomyces coelicolor was cloned and expressed in E. coli. The purified enzyme showed a single protein band on SDS-PAGE with a...
Release of β-galactosidase from Lactobacilli
lactic acid bacteria autolysis β-galactosidase
2009/3/23
Lactobacillus brevis cells at the end of growth release intracellular beta-galactosidase into the medium. Enzyme release begins immediately after the end of cell multiplication and is connected to cel...
Thermomyces lanuginosus CBS 395.62/b Strain as Rich Source of alpha-Galactosidase Enzyme
alpha-galactosidase fermentation enzyme production
2009/3/19
Seventeen Thermomyces lanuginosus strains, cultivated on raffinose and sucrose, were ranked on the basis of alpha-galactosidase activities. T. lanuginosus CBS 395.62/b strain showed the highest alpha-...
Kinetic Properties of α-Galactosidase and the Localization of Total Proteins in Erwinia chrysanthemi
Erwinia chrysanthemi α-galactosidase periplasm
2009/3/19
Erwinia chrysanthemi is an enterobacterium that causes soft-rot in plants in general, resulting in enormous economic losses annually. For the pathogen to survive in the host plant, it has to use the r...
Activity of β-Galactosidase and Polygalacturonase in Zucchini Squash（Cucurbita pepo L.）Stored at Low Temperatures
chilling injury cold signaling oligosaccharins cell wall
2009/2/26
When fruits are exposed to chilling temperatures, enzymatic systems are affected and normal cell metabolism is altered; cell wall enzymes are the least studied in this respect. Our objective is to det...
Optimization of the Culture Medium for the Production of β-Galactosidase from Kluyveromyces marxianus CCT 7082
β-galactosidase Kluyveromyces marxianus optimization
2009/2/24
Seven strains of the genus Kluyveromyces were screened for β-galactosidase activity in a synthetic medium. Kluyveromyces marxianus CCT 7082 was selected as the best enzyme producer for optimization of...
Statistical Optimization of α-Galactosidase Production in Submerged Fermentation by Streptomyces griseoloalbus Using Response Surface Methodology
α-galactosidase response surface methodology Streptomyces griseoloalbus
2009/2/20
α-Galactosidase production by a novel actinomycete strain Streptomyces griseoloalbus in shake flask culture was optimized using response surface methodology. Screening of variables to find their relat...
mmobilization of β-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactose
Immobilization β-galactosidase galacto-oligosaccharides
2009/1/15
A method for immobilization of β-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobiliza...
Kinetic Studies on Endo-β-galactosidase by a Novel Colorimetric Assay and Synthesis of Poly-N-acetyllactosamines Using Its Transglycosylation Activity
endo-β-galactosidase enzyme assay poly-N-acetyllactosamine kinetics transglycosylation
2008/4/20
Novel chromogenic substrates for endo-β-galactosidase were designed on the basis of the structural features of keratan sulfate. Galβ1,4GlcNAcβ1,3Galβ1,4GlcNAcβ-pNP (2), which consists of two repeating...