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鉴于常用的抗体检测方法不能完整反映免疫猪获得针对猪伪狂犬病毒的保护力，有必要建立猪伪狂犬病病毒（PRV）特异性IFN-γ的酶联免疫斑点检测（ELISpot）方法，并评估伪狂犬病疫苗细胞免疫的免疫效果。依照ELISpot基本操作流程，摸索PRV作为刺激原的最适浓度、外周血单个核细胞（PBMC）密度和作用时间以建立并优化该方法。将20头gB抗体检测阴性猪，随机分为单独弱毒疫苗免疫组、单独灭活疫苗免疫组...
Determining the Latent Tuberculosis Infection by IFN - γ Elispot Assay in Healthcare Workers From University Hospitals of Shiraz, South West of Iran
Latent tuberculosis Tuberculin test Interferon - gamma Enzyme-Linked Immunospot Assay Iran
2015/9/30
Background: Classical screening methods are incapable to properly detect LTBI (Latent TB Infection) and HCWs (Healthcare Workers) are at the high risk of exposure. Only few reports estimated the preva...
The comprehensive evaluation of latent tuberculosis infection in health care workers and of patients with active tuberculosis using TST, ELISA, and ELISPOT methods
IFN-g tests QFT-G T-SPOT.TB test tuberculin skin test (TST) latent TB
2010/10/14
Until recently, the tuberculin skin test (TST) has been the only assay used for detecting latent tuberculosis infection (LTBI), but two ex-vivo tests, used as alternative methods to TST, based on enum...
ELISPOT 的技术优势及其在动物免疫研究中的潜力
ELISPOT 免疫检测技术 动物免疫
2009/5/19
介绍了酶联免疫斑点技术( ELISPOT) , 比较了该技术与其他免疫检测技术相比的优势, 展望了ELISPOT 在动物免疫研究中的应用
前景。
用恶性疟原虫红前期多表位候选抗原PfCP-3tcl（含有1个HLA A*0201限制的CTL表位YLNKIQNSL）免疫人白细胞抗原复合体（HLA）A*0201（HLA-A*0201）转基因小鼠，再用鼠γ干扰素（IFN-γ）酶联免疫吸附斑点法（ELISPOT）检测该转基因小鼠特异性CTL应答，尝试在转基因实验动物中建立评价恶性疟原虫红前期候选抗原CTL应答的方法。结果显示该候选抗原中含有的CTL表...