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新乡学院生命科学技术学院基因工程课件 Tools for DNA Cloning。
Molecular cloning and characterization of allele d‒a newly identified allele of the 1-aminocyclopropane-
Malus floribunda 821 polymorphism of ACO1 gene ripening ethylene
2016/6/3
Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This ...
Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Molecular Cloning, mRNA Expression,and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis
Epididymis G-protein Subunit Galphaq [GNAQ] Sheep Testis
2016/12/28
The reproductive function of G-protein subunit Galphaq (GNAQ), a member of the G protein alpha subunit family, has been extensively studied in humans and rats. However, no data is available on its sta...
Cloning and Expression of Yak Active Chymosin in Pichia pastoris
Active Chymosin Yak Expression Pichia pastoris
2016/11/10
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain c...
We investigate the asymptotic relationship between quantum cloning and quantum estimation from the global point of view where all the copies produced by the cloner are considered jointly.For an N-to-M...
We pose the question whether the asymptotic equivalence between quantum cloning and quantum state estimation, valid at the single-clone level, still holds when all clones are examined globally. We con...
Memory-built-in quantum cloning in a hybrid solid-state spin register
Memory-built-in quantum cloning hybrid solid-state spin register
2016/1/22
As a way to circumvent the quantum no-cloning theorem, approximate quantum cloning protocols have received wide attention with remarkable applications. Copying of quantum states to memory qubits provi...
New ‘Performance Cloning’ Techniques Designed to Boost Computer Chip Memory Systems Design
Performance Cloning Boost Computer Chip Memory Systems Design
2015/10/13
Memory Controller and DRAM architecture are critical aspects of Chip Multi Processor (CMP) design. A good design needs an in-depth understanding of end-user workloads. However, designers rarely get in...
Cloning and sequencing of the complicated rDNA gene family of Bos taurus
GC-rich repetitive sequences PCR rDNA rRNA Bos Taurus
2015/6/2
The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcripti...
Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue
Yak Mammary Tissue Milk Lysozyme Recombinant Protein
2016/5/19
Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp ...
Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs
Theileria annulata theileriosis antitheilerials lactate dehydrogenase gene cloning
2015/4/15
Theileriosis is a serious animal disease that is transmitted by ticks. Theileria species are tick-borne obligate intracellular protozoan parasites that cause severe and mild infections in their hosts....
Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis
in vitro ornamental plants somatic embryogenesis
2015/3/3
Protocols were developed for propagation of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea via somatic embryogenesis by manipulating growth regulators and culture conditions. Calli wer...
Cloning and characterization of thioredoxin peroxidase (TPx) in {\it Onchocerca volvulus\/}
Infection silk worm parasites prevent infection animal models and gene
2014/12/11
About 400 million individuals worldwide are currently infected with filarial parasites. To find a cure or prevention for the infections, studies of the mechanisms of pathogenesis and the survival stra...
Cloning and characterization of a new gene involved in lymphocyte activation
The immune system mouse models cow in vitro lymphocyte activation immunology lymphocyte activated gene
2014/12/11
Cattle represent an economically important species whose immune system seems to depart from the standard human and murine models. Little has been documented about bovine lymphocyte activation in vitro...