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Genetic Transformation of a High Molecular Weight Glutenin (Glu-1Dx5) to Rice cv. Fatmawati
Genetic transformation Glu-1Dx5 High molecular weight glutenin Rice
2009/7/21
In order to improve rice dough functionality, we co-transformed the Glu-1Dx5 gene encoding a high molecular weight (HMW) glutenin subunit Dx5 from bread wheat, Triticum aestivum L. and either bar gene...
Genetic Transformation of Coleus blumei Benth.Using Agrobacterium
callus culture Coleus blumei transformation Agrobacterium
2009/3/20
Efficiency in transformation of three hybrids of Coleus blumei using wild and mutant strains of Agrobacterium sp. was evaluated. Successful transformation depended on specific bacterial strain-plant g...
Genetic Transformation of Citrus paradisi with Antisense and Untranslatable RNA-dependent RNA Polymerase Genes of Citrus tristeza closterovirus
Citrus tristeza virus replicase and RNA-mediated resistance plant transformation RNA-dependent RNA polymerase
2009/1/12
Protein and RNA-mediated forms of pathogen-derived resistance (PDR) have been developed against many viruses in different plants. However, no resistance has been reported against Citrus tristeza viru...
甲型肝炎病毒衣壳蛋白融合基因植物表达载体的构建与遗传转化柑桔的研究Construction of the Plant Expression Vector with Hepatitis a Capsid Protein Fusion Gene and Genetic Transformation of Citrus.Sinensis Osbeck
甲型肝炎病毒衣壳蛋白融合基因 柑桔 植物表达载体 遗传转化 食用疫苗
2008/1/20
摘要
基因工程领域的研究进展使得植物体成为具有重要经济价值的药用蛋白的生产体系。以含甲型肝炎病毒结构基因cDNA的克隆载体pCDNAⅡA16为模板，用甲型肝炎病毒衣壳蛋白融合基因特异引物进行PCR扩增，得到全长2.2kb衣壳蛋白融合基因序列。经测序鉴定后正向克隆于植物表达载体pBI121中，衣壳蛋白融合基因位于pBI121质粒T-DNA左右边界区间内，处于CaMV35S启动子控制之下。经限制性内...
Genetic Transformation of Bacillus thuringiensis Insecticidal Toxic Genes to E. Coli Against Spodoptra littoralis (Boisd.)
Transformation Bt isolates E. coli toxicity against S. littoralis larvae plasmid profile and SDS-PAGE
2010/11/9
The aim of this work is to transfer genes responsible for inducing toxin proteins from some local Bt isolates to the gram-negative E. coli strain using genetic transformation. three local Bt isolates ...