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Mullerian inhibiting substance improves longevity of motility and viability of fresh and cryopreserved sperm
motility and viability fresh and cryopreserved sperm
2009/2/18
Mullerian inhibiting substance (MIS) is a hormone present in seminal plasma, but its role is unknown. In this study, the effects of MIS on sperm survival in fresh and cryopreserved specimens were in...
Hormonal regulation of spermatogenesis in the hypophysectomized rat：cell viability after hormonal replacement in adults after intermediate periods of hypophysectomy
Testis testosterone FSH
2009/2/17
A quantitative analysis of germ-cell populations in normal, hypophysectomized (Hx), and Hx-hormone-treated animals was undertaken during periods of regression that were characterized as intermedia...
Hormonal regulation of spermatogenesis in the hypophysectomized rat：FSH maintenance of cellular viability during pubertal spermatogenesis
Testis testosterone hypohysectomy cellviability
2009/2/17
The potential for follicle-stimulating hormone (FSH) to promote germ-cell survival and the cellular sites of FSH action were studied using a gonadally maturing (pubertal), hypophysectomized (Hx) rat...
Improvement of cryopreserved ram sperm heterogeneity and viability by addition of seminal plasma
cryopreserved ram sperm heterogeneity seminal plasma
2009/2/17
The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simulta...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Continuous assessment of human spermatozoa viability during cryopreservation
Sperm cryomicroscopy fluorescent viability probes
2009/2/17
Cryomicroscopy has enabled direct observation of freezing and thawing of human spermatozoa. When used with a fluorescent viability kit, sperm membrane damage was not apparent down to temperatures of...
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide
Sperm fluorescence SYBR-14 flow cytometry vital staining cryopreservation
2009/2/13
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential
Sperm osmotic stress horse cryopreservation glycerol
2008/12/29
Osmotic stress attributed to differences in the relative permeability of cryoprotectants, such as glycerol and water, appears to be an important factor in cryodamage. The objective of this study was...
The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation
reactive oxygen species equine sperm motility viability
2008/12/25
The objective of this study was to examine the influence of reactive oxygen species (ROS), generated through the use of the xanthine (X)-xanthine oxidase (XO) system, on equine sperm motility, via...
Effects of accessory sex gland fluid on viability, capacitation, and the acrosome reaction of cauda epididymal bull spermatozoa
sex gland viability capacitation reaction bull spermatozoa
2008/12/25
The effect of accessory sex gland fluid (AGF) on viability and acrosomal integrity of spermatozoa was examined with cauda epididymal spermatozoa and AGF from the same Holstein bull (n = 6). Surgical...
Protamine Levels Vary Between Individual Sperm Cells of Infertile Human Males and Correlate With Viability and DNA Integrity
Spermatogenesis male fertility
2008/12/24
Sperm protamine deficiency has been associated with human male infertility. However, most studies have adopted a global approach to assessing sperm protamine levels. Thus, it is not known whether...
Relationship Between Sperm Viability as Determined by Flow Cytometry and Nonreturn Rate of Dairy Bulls
Spermatozoa quality insemination fertility precision accuracy
2008/12/23
A newly developed flow cytometric method for determination of sperm concentration and viability was tested in an insemination trial with cryopreserved bull sperm to establish the relationship betwee...
A Flow Cytometric Method for Rapid Determination of Sperm Concentration and Viability in Mammalian and Avian Semen
Cytometry spermatozoa count vitality precision accuracy
2008/12/22
A new flow cytometric method has been developed to rapidly determine sperm concentration and viability in semen from bulls and boars. Sperm viability was determined on the basis of staining with SYB...
Viability and Protein Phosphorylation Patterns of Boar Spermatozoa Agglutinated by Treatment With a Cell-Permeable Cyclic Adenosine 3',5'-Monophosphate Analog
Serine/threonine phosphorylation tyrosine phosphorylation hyperactivation
2008/12/19
Boar spermatozoa become agglutinated with one another at the head when their intracellular cyclic adenosine 3',5'-monophosphate (cAMP)-signaling cascades are activated in the head. The aim of the ...