搜索结果: 1-15 共查到“医学遗传学 PCR”相关记录33条 . 查询时间(0.149 秒)
转Cry1Ab基因水稻Bt01为一种新型的转基因水稻, 文章首先利用Southern blotting验证了外源基因Cry1Ab转入了Bt01中, 且为单拷贝, 再利用TAIL-PCR方法获得了其插入位点信息, 根据获得的Bt01的5′端插入位点序列, 设计了相应的定性与定量PCR检测体系的引物及探针, 实验结果显示, 定性PCR检测体系的最低检测极限(LOD)为10个拷贝, 定量PCR检测体系的...
以番茄(Solanum lycopersicum L. cv. Micro-Tom)叶片为试材, 建立了一种简便快速制备叶片基因组DNA的方法。2~20 mm2的叶片即可满足制备要求, 制备过程只需一种提取试剂、只涉及1次移液和1次离心操作, 不涉及沉淀。确定了所制备的DNA用于实时荧光定量PCR的合适用量为0.1~0.2 mL (反应总体积为12.5 mL), 发现过量模板的使用可降低PCR效率...
为评估定量荧光PCR (QF-PCR)方法在男性不育遗传学诊断中的应用价值, 文章对78例非梗阻性男性不育患者, 采用精液常规检测精子情况, 并检测患者性激素水平; 采用QF-PCR方法对患者性染色体多态性STR位点及特异性位点进行检测; 采用常规染色体G显带方法进行核型分析; PCR检测AZF微缺失。结果显示78例非梗阻性男性不育患者中发现无精子症患者18例, 少精子症患者20例, 总检出率为4...
Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran,Iran Using PCR-RFLP
HBV Hbs Ag PCR-RFLP Blood Donors Iran
2009/12/17
Background: Hepatitis B virus (HBV) is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a...
Detection of the SRY Gene in a 46,XY Phenotypic Female by the PCR-SSCP Method
PCR-SSCP Sex reversal SRY 46 XY Female
2009/6/30
The short arm of the human Y chromosome determines maleness, initiating the development of the testes. Mutations and deletions in this region affect gonadal differentiation, causing primary sex revers...
Quantification of The FLI1 Gene Expression By Real-Time Quantitative RT-PCR
Gene expression FLI1 PML-RARA Real Time RT-PCR
2009/6/25
In this study, quantification levels were investigated to define alterations in the expression of the FLI1 gene on acute promyelocytic leukemia (APL), which is characterized by a reciprocal t(15,17) t...
PCR Analysis of HSV-Negative Erythema Multiforme for the Expression of Other Herpesviruses
Erythema multiforme Epstein-Barr virus cytomegalovirus human herpesvirus-6 human herpesvirus-7 polymerase chain reaction
2009/6/25
Background: Herpes simplex virus (HSV) is the primary herpesvirus implicated to have a causal role in erythema multiforme (EM), both in cases with an antecedent herpetic infection and in idiopathic EM...
Expression Analysis of DEK, AF4 and FLI1 Genes in All-Trans-Retinoic Acid (ATRA) Treated Acute Promyelocytic Leukaemia t(15;17) Patients by Quantitative Real-Time PCR
DEK AF4 FLI1 Acute Promyelocytic Leukemia All-Trans-Retimic Acid
2009/6/23
All-trans retinoic acid (ATRA) sensitivity of acute promyelocytic leukaemia (APL) cells is strictly dependent on the presence of t(15;17), but the molecular background of this sensitivity remains obsc...
Prenatal Diagnosis of a Trisomy 13 Case Associated with Holoprosencephaly by Ultrasonography and Quantitative Fluorescent PCR
Aneuploidies holoprosencephaly QF-PCR prenatal diagnosis trisomy 13 ultrasonography
2009/6/22
Trisomy 13, first described by Patau in 1960 (1),occurs in 1/5000 of births and is the most severe of the autosomal trisomies (2). Common features of trisomy 13 include holoprosencephaly with midfacia...
Evaluation of the Efficacy of Five DNA Extraction Methods for the Detection of Mycobacterium tuberculosis DNA in Direct and Processed Sputum by an In-House PCR Method
DNA extraction Mycobacterium tuberculosis PCR
2009/5/20
Aim: DNA extraction is an important step from clinical samples for molecular diagnosis of tuberculosis by PCR. The aim of this study was to evaluate the efficacy of five DNA extraction methods [boil...
The efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of prenatal aneuploidy
Aneuploidy QF-PCR conventional karyotype analysis
2010/1/11
Aim: To investigate the efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of aneuploidy. Materials and methods: The study included 40 pregnant women considered t...
综述检测CYP2A6基因多态性的PCR技术的基因型 方法。CYP2A6基因多态性存在 于白种人、东方人及非洲裔美国人群中，包括CYP2A6*1至CYP2A6*12, 总共12个变异等位基因。 本文列举了检测CYP2A6基因型的不同的DNA来源、引物和限制性 内切酶。已开发PCR技术结合 诊断性限制性内切酶分析（RFLP）如两步PCR和一步PCR-RFLP方法检测CYP2A6基因型。此外，尚有PCR...
等位基因特异PCR 检测N-乙酰化转移酶遗传多态性研究
等位基因特异PCR 遗传多态性 N2乙酰化转移酶
2008/9/12
目的与方法:改良一种适用于流行病学研究的N2乙酰化转移酶(NAT2) 基因型的等位基因特异PCR(AS2PCR) 检测方法,分析了3 个NAT2 基因突变点:481 T、590 A、857 A ,并用此法对76 例南京地区健康人群的NAT2 基因型多态性进行了研究。从而也检验了该方法。结果:该人群中各基因频率:Wt (野生型) 为0. 5723 :481 T 为0. 0395 ;590 A 为0....
本文对等位基因特异性PCR(Allele-specific , AS2) 和多重差别(Multiplex differential , MD2) PCR 技术进行了优化,并用此法联合检测了105 例江苏地区健康人群及68 例肺癌患者CYP1A1、GSTM1 的等位基因型。结果表明:AS2PCR 及MD-PCR采用的设立双参照扩增体系,可一次同时检测CYP1A1 和GSTM1 的等位基因型。在肺癌患...