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Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Myostatin Zinc-finger Nucleases Knockout, Sheep
2016/11/10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause “double-muscling” trait in animals. In order to block the inhibiting effect of M...
用PCR-RFLPs方法对南阳牛、秦川牛、郏县红牛3个品种共411个个体的myostatin 5′调控区的单核苷酸多态性(SNPs)进行分析。结果表明，3个品种在myostatin 5′调控区T→A突变以等位基因T为主，仅在郏县红牛中发现1个AA型纯合体，郏县红牛品种显著偏离Hardy-Weinberg平衡状态(P<0.05)，其它品种处于Hardy-Weinberg平衡状态(P>0.05)。对黄...