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The Effect of Treating Forages with Fibrolytic Enzymes on its Nutritive Value and Lactation Performance of Dairy Cows
enzymes cellulase xylanase forage
2008/7/16
Forages (corn silage and alfalfa hay) were sprayed with liquid enzymes prior to combining with a concentrate to form a total mixed ration (50% forage:50% concentrate, dry matter basis) and fed to lact...
Time Trends of Plasma Lipids and Enzymes Synthesizing Hepatic Triacylglycerol During Postpartum Development of Fatty Liver in Dairy Cows
dairy cows fatty liver dry period glycerolipid synthesis
2008/7/2
We studied development of fatty liver in high producing dairy cows with free access to feed during the dry period and thus showed the combined effects of parturition and prepartum overfeeding. Postpar...
Food Biochemical Study on Fructans and Related Synthesis Enzymes
fructan fructo-oligosaccharide fructosyltransferase post-harvest Pichia pastris
2008/4/20
There are more than 500 kinds of oligosaccharide occurring in nature, and all of these are synthesized by chemical and enzymatic reactions. Various studies have recently identified physiological and p...
Fungal Exo-acting Enzymes with Novel Catalytic Properties
exo-polygalacturonase xylogalacturonan acetylated homogalacturonan exo-arabinanase arabinobiose
2008/4/20
This article deals with characterizations of two Aspergillus niger exo-polygalacturonases (exo-PGs; EC 3.2.1.67) and a Penicillium chrysogenum exo-arabinanase (no EC number). Two exo-PGs, termed exo-P...
Carbohydrate-active Enzymes from Extremophiles
alkaliphiles Bacillus cellulase amylase pectate lyase detergent enzyme adaptation
2008/4/20
The term “extremophiles” is used for organisms that thrive under extreme conditions, and are designated the enzymes that are active and tolerant under extreme reaction conditions “extremozymes”. We ha...
Developing and Engineering Enzymes for Manufacturing Amylose
amylose glucose polymer α-glucan phosphorylase glycogen debranching enzyme protein engineering
2008/4/20
Amylose is a functional biomaterial and is expected to be used for various industries. However at present, manufacturing of amylose is not done, since the purification of amylose from starch is very d...
Pectin-degrading Enzymes Produced by Fungi
pectin arabinanase ferulic acid esterase rhamnogalacturonase exo-polygalacturonase
2008/4/20
Carbohydrates are the most abundant material produced by plants and processes for their efficient conversion and utilization are of major importance in biotechnology. Their enzymatic conversion can be...
Properties and Application of Enzymes for Bacterial Glycogen Biosynthesis and Degradation
branching enzyme cyclization dextrin glycogen ADP-glucose pyrophosphorylase
2008/4/20
Structures and properties of enzymes for bacterial glycogen metabolism have been investigated. Branching enzyme (BE, EC 2.4.1.18), which is responsible for α-1,6 glucosidic linkages of glycogen, was f...
Engineering Thermus Maltogenic Amylase with Improved Thermostability: Probing the Role of the Conserved Calcium Binding Site in Cyclodextrin-degrading Enzymes
calcium binding site cyclodextrin-degrading enzymes mutagenesis thermostability Thermus maltogenic amylase
2008/4/20
Thermus maltogenic amylase (ThMA), one of the cyclodextrin (CD)-degrading enzymes, is expected to have a calcium-binding site (Ca2 site) based on multiple sequence alignments. In spite of the presumpt...
N-Linked Oligosaccharide Processing Enzymes as Molecular Targets for Drug Discovery
α-glucosidase ER glucosidase inhibitor anti-viral activity
2008/4/20
N-Linked oligosaccharide processing enzymes are key enzymes in the biosynthesis of N-linked oligosaccharides. These enzymes are a molecular target for inhibition by anti-viral agents that interfere wi...
Reaction Mechanism and Substrate Recognition of GH-94 Phosphorolytic Enzymes
crystallography GH-94 phosphorolytic enzyme reaction mechanism structure and function relationship
2008/4/20
Vibrio proteolyticus chitobiose (GlcNAc-β1,4-GlcNAc) phosphorylase (ChBP) catalyzes the reversible phosphorolysis of chitobiose into α-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric conf...
Studies on Functional Analysis of Plant Starch Biosynthetic Enzymes
starch biosynthesis starch branching enzyme starch synthase subcellular localization
2008/4/20
About three centuries have already passed since Antoni van Leeuwenhoek observed the gelatinization of starch granule under a microscope in 1719. Now, many people know not only the word “starch,” but a...
Study of Trehalose-relating Enzymes
trehalose trehalase trehalose synthase trehalose phosphorylase
2008/4/19
Trehalose (α-Glcp-(1↔1)-α-Glcp) is widely distributed in nature such as microorganisms, insects, plants, and invertebrates. This sugar exists not only as an energy source but also as an importan...
Immediate Effect of Lactobacillus acidophilus on the IntestinalFlora and Fecal Enzymes of Rats and the In Vitro Inhibitionof Escherichia coli in Coculture
Lactobacillus acidophilus ß beta -glucuronidase
2008/4/1
The in vitro role of Lactobacillus acidophilus was investigated to explore the potential to inhibit coliforms. A threefold concentrated cell-free extract from L. acidophilus SBT2074 could efficiently ...
ß-Casomorphins: Analysis in Cheese and Susceptibility to Proteolytic Enzymes from Lactococcus lactis ssp. cremoris
ß beta -casomorphins cheese proteolysis Xprolyl dipeptidyl aminopeptidase
2008/3/25
Commercial cheese products were surveyed for ß-casomorphin peptides. Two extraction methods were compared: 1) water and 2) chloroform and methanol. Peptide profiles were determined using reverse...