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The process parameters for non-typical seeds during simulated cold deep oil expression
oil seeds fat content cold expression oil-point
2014/2/25
We have determined the parameters of oil expression process for non-typical seeds of oil-producing plants, such as quince tree, safflower, fennel-flower, cuckoo-flower, tarweed, lallemantia, sea-buckt...
Heterologous Expression of Xylanase II from Aspergillus usamii in Pichia pastoris
Aspergillus usamii characterization expression xylanase Pichia pastoris
2009/6/24
To efficiently produce xylanase for food processing industry, a gene encoding xylanase II (XynII) from Aspergillus usamii has been cloned into the vector pPIC9K and integrated into the genome of Pichi...
Effects of the White-Skinned Sweet Potato (Ipomoea batata L.) on the Expression of Adipocytokine in Adipose Tissue of Genetic Type 2 Diabetic Mice
white-skinned sweet potato adiponectin adipocytokines
2009/6/10
We investigated the effects of white-skinned sweet potato (WSSP) on the expression of adipose tissue adipocytokine in order to elucidate the mechanisms underlying the anti-diabetic effects of WSSP. A ...
Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
α-galactosidase Streptomyces coelicolor A3(2) glycoside hydrolase
2009/6/10
An α-galactosidase gene belonging to glycoside hydrolase family 27 from Streptomyces coelicolor was cloned and expressed in E. coli. The purified enzyme showed a single protein band on SDS-PAGE with a...
Constitutive Expression and Inducibility of Plasminogen Activator in Mer- Glioblastoma A1235 Cell Line and in Mer+ A8 Cells Transfected with Bacterial ada Gene
A1235 human glioblastoma Mer phenotype MNNG
2009/3/23
The alkylation repair deficient (Mer- phenotype) cells produce higher levels of proteolytic enzyme plasminogen activator (PA) after treatment with alkylation agent N-methyl-N'-nitro-N-nitrosoguanidine...
The in vivo Expression of Streptomyces rimosus tRNA Genes
Streptomyces rimosus expression promoter
2009/3/23
The expression of seven tRNA genes from Streptomyces rimosus, cloned on bifunctional plasmids, has been studied in a homologous (S. rimosus) and a heterologous (Escherichia coli) system. Analyzed gene...
Gene Function and Expression: Four Years of the Post-genomic Era of Yeast
gene expression gene function genomics
2009/3/23
The yeast Saccharomyces cerevisae is a key model organism in which eukaryotic cell architecture and fundamental cellular mechanisms can be successfully investigated. The yeast genome sequence programm...
Effect of Growth Conditions on the Expression of Soluble Methane Monooxygenase
soluble methane monooxygenase type II methanotroph methanotrophic-heterotrophic community
2009/3/20
Methanotrophic-heterotrophic groundwater community (culture MM1) and the obligate type II methanotroph (strain Met1) isolated from the culture MM1 were found to express soluble methane monooxygenase (...
Curcumin - A Potent Inhibitor of Galectin-3 Expression
galectin-3 curcumin UV light alkylating agent MNNG
2009/3/20
The expression of galectin-3, a β- galactoside binding lectin, was found to be affected by different kinds of stressors, and is strongly modified in numerous physiological and pathophysiological condi...
Effects of Structurally Related Flavonoids on hsp Gene Expression in Human Promyeloid Leukaemia Cells
hsp genes flavonoids leukaemia
2009/3/20
Quercetin is a known specific inhibitor of hsp70 synthesis and thus might be a potent agent for enhancing the selective cytotoxicity of heat on tumour cells. A comparative analysis of the effects of q...
Fungal Invertase Expression in Solid-State Fermentation
invertase solid-state fermentation expression kinetics
2009/3/11
In this study invertase activity expression in Aspergillus niger Aa-20 was evaluated under different concentrations of two substrates using solid-state fermentation (SSF) on polyurethane foam. Glucose...
Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent
tannase Pichia pastoris recombinant protein production
2009/2/25
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of As...
Integrative Expression of Glucoamylase Gene in a Brewer’s Yeast Saccharomyces pastorianus Strain
glucoamylase brewer’s yeast diacetyl fermentation
2009/2/23
The recombinant brewer’s yeast Saccharomyces pastorianus strain was constructed by introducing the ilv2::GLA fragment released from pMGI6, carrying glucoamylase gene (GLA) and using the yeast α-acetol...
Spatial and Temporal Expression of Transferrin Gene In the Rat Mammary Gland
rat mammary transferrin in situ hybridization
2008/7/31
The distribution and concentration of transferrin mRNA in the rat mammary gland was analyzed using in situ hybridization, and the results were compared with those obtained for other milk protein mRNA....
Expression of a Cellobiohydrolase cDNA from the White Rot Fungus Irpex lacteus in Aspergillus oryzae
cellobiohydrolase Irpex lacteus heterologous gene expression
2008/4/20
A cDNA (cel2) coding for an exo-type cellobiohydrolase was isolated from the basidiomycete Irpex lacteus strain MC-2. The cel2 cDNA was expressed in a heterologous host Aspergillus oryzae by using an ...