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Interactions between Barley α-Amylases, Substrates, Inhibitors and Regulatory Proteins
glycoside hydrolase family13 secondary binding sites surface plasmon resonance calcium effects regulatory proteins
2008/4/20
Barley α-amylase binds sugars at two sites on the enzyme surface in addition to the active site. Crystallography and site-directed mutagenesis highlight the importance of aromatic residues at these su...